Abstract
Medicinal plants possess certain therapeutic properties including synergistic actions. Plant extracts and individual biological active compounds play a crucial role in the treatment and prevention of different diseases. Recently, much attention has been paid to research aimed at finding antimicrobial agents among medicinal plants used in folk medicine. According to the World Health Organization (WHO), the problem of antimicrobial resistance is a global threat to public health and health development. Thus, the need to create new antimicrobial agents of plant origin determines the relevance of the study. In this regard, the purpose of this study is to study the component composition and antimicrobial activity of the ethanolic extract of Portulaca oleracea L. The methods of Gas-Chromatography–Mass Spectrometry (GC–MS) and High-performance liquid chromatography (HPLC) were used to determine the component composition. Antimicrobial activity was studied by the method of two-fold serial dilutions using test cultures of microorganisms Staphylococcus aureus ATCC 6538-P, Streptococcus pneumoniae ATCC BAA-660, Acinetobacter baumannii ATCC 1790, Bacillus subtilis ATCC 11778, Escherichia coli ATCC 8739, Candida albicans ATCC 10231, and Candida albicans - clinical isolate. Based on the results of GC-MS analysis, 35 compounds were identified in the ethanolic extract, and organic compounds as Catechin, Epicatechin, Naringin, Phloridzin were detected by HPLC analysis. The research results showed that the ethanolic extract of Portulaca oleracea L. it has antimicrobial activity (Bactericidal, fungicidal, sporocidal) against all tested museum strains of microorganisms. Thus, the ultrasonic ethanolic extract of Portulaca oleracea L. is promising for the pharmaceutical industry, has great potential for antimicrobial activity against pathogenic bacteria.