Abstract
This study focused on examining how plant growth regulators affect shoot proliferation on Crataegus sanguinea. Shoot multiplication treatments included benzylaminopurine (6-BAP) at different concentrations (0.25, 0.5, and 0.75 mg/l) combined with constant 0.5 mg/l gibberellic acid (GA3) and 0.01 mg/l Indole-3-butyric acid (IBA), in Quoirin & Lepoivre (QL) nutrient medium. The results showed that the highest number of shoots per explant (5.23) was achieved in a medium supplemented with 0.5 mg/l 6-BAP plus 0.5 mg/l GA3 and 0.01 mg/l IBA, while the greatest shoot length (12,77 cm) was obtained with 0.75 mg/l 6-BAP plus 0.5 mg/l GA3, and 0.01 mg/l IBA 35 days after transplantation. This study presents an efficient protocol for in vitro multiplication of Crataegus sanguinea, achieving the highest shoot proliferation rate.