VERIFICATION OF ANALYTICAL METHODS IN THE ANALYSIS OF RHODIOLA HETRODONTHA (HOOK. F. ET THOMSON) BORISS

2025

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DOI 10.53511/pharmkaz.2025.95.68.037
Data confidence 70 medium
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Abstract

The article presents the verification of pharmacopoeial identification and quantitative determination methods as applied to the raw material of Rhodiola hetrodontha. Characteristic of the genus Rhodiola SPP. plant phenolic compounds such as salidroside and tyrosol were determined in the rhizomes and the roots of Rhodiola hetrodontha, and rosarin was not identified. The verification of the pharmacopoeial method for the quantitative determination of genus-specific phenolic compounds in the rhizomes and roots of Rhodiola hetrodontha was carried out, which consists of the extraction of phenolic compounds with 70% ethyl alcohol and analysis by high-performance liquid chromatography. The optimality of the pharmacopoeial conditions for chromatographic analysis (HPLC and FETLC) of genus-specific phenolic compounds in the rhizomes and roots of Rhodiola hetrodonta has been confirmed. The specificity, linearity, correctness, accuracy and range of application of the studied methods have been proven. Therefore they can be used in quality control of the rhizomes and roots of Rhodiola hetrodonta.