Abstract
High concentrations of gonadotropic serum were primarily found in the mare’s blood serum. Consequently, the amount of serum obtained after blood collection from pregnant mares depends on the volume of blood collected, which increases the chances of obtaining a greater quantity of gonadotropic hormones. Therefore, the effectiveness of these methods for isolating gonadotropic serum from the blood of pregnant mares was investigated. It was found that the most effective method for isolating gonadotropic serum from the blood of pregnant mares is the use of 5% and 7.5% citric acid solutions. By introducing a new technology into agricultural biotechnology that accelerates serum release, the volume of hormonal serum with high gonadotropic activity increased on average by 55.8 ± 0.5%, showing a 9.1 ± 0.9% improvement over traditional methods. For the first time, a procedure for using citric acid solution has been incorporated into the technology for extracting serum from the blood of pregnant mares. This improved approach accelerates the extraction process and increases the serum volume by 1.7 ± 0.1ml. Gonadotropic activity of the blood serum obtained using various solutions was studied. It was found that the solutions used to isolate serum from the blood of pregnant mares have a significant impact on the activity of the resulting hormonal preparation, with serum activity ranging from 144.5 IU/ml to 244.7 IU/ml. The gonadotropic activity of blood serum obtained using a general citric acid solution ranged from 80.5 IU/ml to 100.2 IU/ml, compared to the solution of acetic acid and sodium chloride. These studies demonstrated the advantage of using citric acid solutions for the efficient and rapid extraction of gonadotropic hormones from the blood of pregnant mares.