Abstract
Bone defects caused by open fractures, oncological diseases and chronic osteomyelitis remain an urgent problem in Traumatology and orthopedics. Currently, many experimental bone defect samples have been developed in laboratory animals in Traumatology. Using world experience, we decided to use a pattern that is made on rabbits to create a pattern of a bone defect and cover it.The purpose of the study is to conduct clinical, radiological and histological analysis of various samples of bone defect coverage in seedless rabbits.Methods. All research was carried out in accordance with the requirements of the bioethics Commission. Laboratory research was carried out on 60 seedless rabbits. They were randomly divided into 2 groups. Rabbits were kept in a vivarium with specialized ventilation, temperature and humidity. Laboratory animals were placed in special cages from 2. All surgical interventions were carried out under general anesthesia. Surgical access was made to the outer surface of the right midsection. In all animals, depending on the group, the defect is filled with growth factors and bisphosphonate.The process of filling and restoring the defect was evaluated by clinical, radiological, histological and statistical methods.Results. Due to the early stage of Ota, low motor activity was observed in the first two days. During forced movement, rabbits limped to the operated jaw and often spent a lot of time in a sitting position. During the visual examination, decreased appetite, general weakness were noted, but the temperature indicators were normal from the beginning of the observation until they were excluded from the experiment. Evaluation of the Radiological picture of the formed samples of bone defects after 14 days, a pronounced periosteal reaction was observed in 2 groups. A comparative description of histological changes in bone tissue within 14 days after surgical treatment was made. To assess the cell composition, the material is colored with hematoxylin and eosin according to the standard method. For histological differentiation of cellular and non-cellular elements of connective tissue, Trichrome staining was performed according to Masson, which made it possible to assess the tendency to fibrosis.Conclusion. The bottom line is that bone defects are still a serious problem and their treatment presents considerable difficulties. Obviously, more research is needed in this area to deepen our understanding and improve treatment outcomes.