Study of enzymatic changes in model object Brachypodium distachyon as part of its non-specific resistance to leaf rust

2022

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DOI 10.1055/s-0042-1759094
Достоверность данных 70 средняя
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Аннотация

According to CIMMYT, wheat provides 20% of the daily protein for 4.5 billion people. By 2050 the demand for wheat is expected to increase by 60%, which requires about 1.6% of annual wheat yield increase. At the same time, about 40% of food crops are annually lost due to plant pests and diseases. One of the key pathogens affecting wheat in Kazakhstan is leaf rust. With severe damage to plants, in the ears, fewer grains are formed; they are of low quality, lightweight, which is the main cause of the crop shortage (up to 40 – 60% in the years of epiphytothies). In our research we used inbred lines of the wild wheat relative Brachypodium distachyon : Bd21, Bd 3 – 1, Bd 1 – 1 for a comparative study of non-specific resistance of the model plant to leaf rust. In particular our studies have shown that on the 3rd day after infection of plants of the Bd21 line, peroxidase (one of the most important catalysts among biotic factors protecting plants from phytopathogens) activity increases by 50%, Bd3-1 by 35%, Bd1-1 by 16.8% relative to the control, which is possibly associated with an increase in the content of Н2О2 in the cells and non-specific response of plants to infection. Another interesting finding was related to inhibition of XDH activity possibly related to increased resistance of Bd21. Some anatomical changes have also been observed, including increased indices of internal structures associated with a protective response to the pathogen damage. Research was funded by АР05 134 104 project (MES RK). Publication History Article published online: 12 December 2022 © 2022. Thieme. All rights reserved. Georg Thieme Verlag KG Rüdigerstraße 14, 70469 Stuttgart, Germany