Аннотация
Detoxification and antioxidant functions of liver of marsh frog (Ranaridibunda) exposed to water-soluble fraction of oil from Kenkiyak oilfieldin concentrations of 0.05, 0.5 and 1% during 30 days were studied. Dosedependent increase of cytochrome P450 content (1.5-2 times) in comparison to intact animals (control), indicating the activation of detoxificationprocesses in liver of intoxicated frogs was observed. It was shown that thegrowth of monooxigenases was accompained by enchancement of lipidperoxidation processes: the content of MDA in liver of intoxicated marshfrogs also rose dose-dependently 1.5-2 times(Р≤0,05)compared with control. Herewith, the content of reduced glutathione, one of the key substances of antioxidant and detoxification systems of the body, decreased1.3-2 times (Р≤0,05) as a result of almost the same induction ofglutathioneS-transferase (1.3-2.1 times, Р≤0,05). It was found that activation of lipidperoxidation in liver of intoxicated marsh frogs was caused by supressionofactivity of catalase (1.2-1.5 times) and superoxide dismutase (1.1-1.4times).On the basis of obtained results a conclusion about activation of detoxification functions and inhibition of antioxidant defence in liver of frogs,leading to strengthening of oxidative stress in studied animalsas a result ofoil intoxication, was made. Accumulation of lipid peroxides in liver led todisruption of adaptive reactions of intoxicated animals and developmentof destructive and necrobioticchanges of hepatocytes.Key words: marsh frog, liver, oil, cytochrome P450, malondialdehyde,reduced glutathione, glutathione-S-transferase, catalase, superoxide dismutase.