Аннотация
Aspergillus oryzae M was cultivated for 42 days in submerged conditions of growth using new method of fungal cultivation.This method based on immobilizing enzymes producers on solid career in submerged conditions of growth allows to prolong the process of fungal cultivation and to maintain high enzymatic activity for a long period of time.To identify the active isolate formed on the deep fungal mycelium, a selection of a number of isolates was carried out at different periods of submerged cultivation.Aspergillus oryzae M/4 was selected as active isolate with improved extra cellular -amylase activity.