Аннотация
A method for the quantitative determination of atorvastatin (ATV) and glycyrrhizic acid (GK) as part of a combined drug in tablet form by high-performance liquid chromatography (HPLC) with diodematrix detection has been developed and validated. Chromatographic separation was performed on a Poroshell C18 column (Agilent 1260) using a mobile phase acetate buffer (0.05 M, pH 5.0) – acetonitrile and diode matrix detection. The quantitative content of the components is calculated using the external standard method. According to the results of five repeated measurements (n=5), the average ATP content found was 8.734 mg (87.34% of the claimed 10 mg) with RSD of 1.16%, the HA content was 46.27 mg (92.55% of the claimed 50 mg) with RSD of 1.21%; the relative error of the average result did not exceed ~ 2%. The methodology is validated in terms of specificity, linearity, precision (repeatability), correctness and reproducibility; the results obtained comply with the requirements of the State Pharmacopoeia of the Republic of Kazakhstan and the Pharmacopoeia of the EAEU.