Аннотация
Introduction. Elite athletes undergo regular high-intensity training to maintain fitness and improve performance. Training overload is necessary to achieve significant results. However, failure to balance training and recovery can lead to overtraining syndrome. To prevent this, regular monitoring of biochemical markers is required to assess the body's adaptive processes. Aim of the study. Comparative analysis of blood biochemical parameters in Olympic athletes of Kazakhstan to assess training load effects and prevent overtraining syndrome Methods. A biochemical analysis was conducted to measure levels of creatinine, creatine kinase (CK), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) in Olympic athletes. Results. Significant differences in biochemical markers were observed among sports disciplines. Creatinine (p<0.001), alkaline phosphatase (p<0.001), AST (p<0.001), CK (p=0.008), and LDH (p<0.001) levels differed significantly between groups, whereas ALT showed no significant variation (p=0.191). Post hoc analyses revealed higher creatinine levels in weightlifters and freestyle wrestlers, elevated alkaline phosphatase levels in combat sport athletes, and significant sport-specific differences in AST, CK, and LDH, reflecting variations in muscle metabolism and training-related physiological adaptation. Conclusion. These findings underscore the importance of monitoring biochemical markers in elite athletes to prevent overtraining and optimize the training process. Keywords: biochemical markers, sports medicine, creatine kinase, lactate dehydrogenase, athletes of Kazakhstan.