Аңдатпа
Abstract Colorectal cancer is one of the three most common oncological diseases worldwide and has a high mortality rate. The complexity of early diagnosis of the disease lies in its polygenic nature. Colorectal cancer is accompanied by a change in the concentration of nanoscale miRNAs (mRNA-inhibiting RNA), which can alter the expression of candidate genes associated with the disease. The purpose of this study was to identify the interactions between 6274 human miRNAs and 28 mRNAs (messenger RNA) of colorectal cancer candidate gene. The quantitative characteristics of these interactions were determined using the MirTarget program. The binding sites of 142 miRNAs in 28 candidate gene mRNAs were determined. 28 miRNAs and mRNA genes associations in 5′UTR (5′-untranslated region), ten in CDS (coding sequence) with a free energy of interaction more than -130 kJ/mol, and multiple binding sites clusters of ID00436.3p-miR, ID01030.3p-miR, miR-466 and ID00470.5p-miR, miR-574-5p are recommended for the diagnosis of colorectal cancer. Significant differences were found in the characteristics of the miRNA interactions in the 5′UTR, CDS and 3′UTR (3′-untranslated region) of mRNA candidate genes. The features of the miRNA binding sites have been established depending on their location in the 5′UTR, CDS and 3′UTR. The miRNA binding sites with overlapping nucleotide sequences that form clusters were identified. The organization of binding sites on clusters leads to compaction and competition between miRNAs for binding in the cluster. The most effective associations between miRNAs and candidate target genes, which are proposed as markers for the development of methods for the early diagnosis of colorectal cancer are determined.