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Recent advances in plant genetic engineering and innovative applicationsPlant genetic engineering is one of the most popular advances in plant science and has in recent years become part of mainstream discussion in society (Mackelprang and Lemaux, 2020).This is born out of multiple unresolved debates on genetically modified organisms (GMOs), the involvement of policymakers in an attempt to regulate and ensure its safe application to food crop production, and it also being featured as a topic in the politics of global aid (Steinwand and Ronald, 2020).Apart from the GMO debate, however, several advances in genetic engineering have been recorded that empower plant scientists to investigate and solve previously unexplored problems (Evanega et al., 2022).From the challenge of transforming recalcitrant species and the development of genetic engineering techniques suitable for non-angiosperms to the development of novel genetic engineering technologies and updates to existing technologies, the field of plant genetic engineering is growing and extending the limits of possibilities in Plant Sciences (Zhang et al., 2019).The extension of plant science research capabilities is particularly important as Plant Science plays a significant role in global hot topics such as climate change and sustainability.The goal of this Research Topic is to highlight studies that embody these advances in new biotechnological tools (NBTs) development and the innovative applications of plant genetic engineering.Studies that focus on NBT development for recalcitrant or previous non-transformable species to allow the unlocking of the biology of these species are of significant interest to this collection.Furthermore, the application of novel strategies of next-generation genetic engineering technologies such as genome/gene editing and protein-domain specific technology (e.g., K-Domain technology) (Song and Han, 2021) and innovative applications as well as updates on well-established genetic Frontiers in Plant Science frontiersin.