Аңдатпа
The results of studying hybrids and lines of sugar beet resistant to unfavorable factors of crop cultivation are presented. The study was carried out on 50 samples of domestic and foreign selection from various countries of the world:Russia,Ukraine,Kyrgyzstan,Germany,Austria. The experiment was carried out in laboratory conditions be means of germination methods at low temperatures and restoration of regeneration processes using in vitro culture. The assessment of the resistance of sugar beet genotypes to cold stress was carried out by physiological method of seed germination at a temperature of4°Cin a climatic chamber during 45-48 days. Samples showing high germination ability were identified: ChS 97 (50%), Kirgizskaya 069 (42), ChS 1631 (38), Biyskaya 32 (38), PMC 133 (33), Uspekh (31), Ramonskaya 125 (30%). These forms are recommended for cultivation in the northern regions of theRepublicofKazakhstan. The assessment of cold resistance using in vitro culture was carried out according to the methodology developed by theInstituteofBioenergy Cropsand Sugar Beets (Kiev,Ukraine). Hypocotyls with apical buds (petioles) of 15-day-old seedlings of sugar beet hybrids were used as explants. Based on the assessment of collection samples of sugar beet using in vitro culture, the following genotypes were selected: Kirgizskaya 069, ChS 97, PMC 60, ChS 1611, 2249; ChS 97 and ChS 1611 lines. These samples, capable of restoring regeneration processes after prolonged cold stress at temperature4°C, were microclonally propagated in order to be included in the breeding process with the purpose of obtaining cold-resistant hybrids.