Аңдатпа
Objective: This study aimed to assess the acute and subacute toxicity of medicinal agent based on the association of probiotic lactic acid bacteria Lacticaseibacillus rhamnosus 7n, Lacticaseibacillus parasasei B-3/43, Lacticaseibacillus casei 14d/13 and 1% alcohol extract of licorice (Glycyrrhiza glabra). Method: Laboratory mice and rats were selected for the study, and the medicinal agent was administered orally and intraperitoneally. Result: Based on the studies conducted on determination of acute toxicity with single oral administration to mice at doses of 25 mg/kg, 250 mg/kg, 2500 mg/kg and 5000 mg/kg and to rats at doses of 27.8 mg/kg, 278.0 mg/kg, 1111.2 mg/kg and 2222.4 mg/kg of weight, and with intraperitoneal administration to mice at doses of 2.5 mg/kg, 25.0 mg/kg, 250.0 mg/kg, 2500 mg/kg and to rats at doses of 2.8 mg/kg, 27.8 mg/kg, 278.0 mg/kg, 555.6 mg/kg of weight, it showed a favorable safety profile without signs of toxicity. When studying the subacute toxicity in laboratory mice at doses of 25.0 mg/kg, 250.0 mg/kg and 2500.0 mg/kg, no toxic effect on the body was revealed. Conclusion: The investigated substance can be classified as non-toxic/harmless. No biological reactivity or deaths were observed in animals during 14 days of acute toxicity assessment and 28 days of subacute toxicity assessment after administration of the tested solutions at various doses. The somatic state remained stable. Analysis of the body weight of animals showed no statistically significant differences between the experimental groups. In all groups, a positive increase in body weight was observed. A comparison of the absolute and relative masses of organs between the experimental groups showed no significant differences. Macroscopic examination revealed that anatomical location, morphological characteristics and appearance of the organs corresponded to the physiological norms characteristic of this species.The calculation of the average lethal dose (LD₅₀) is impossible due to the absence of fatal outcomes.