Аңдатпа
Background: Biodegradable nanoparticles (NPs) are popular drug delivery system because of their capability to revert to non-toxic byproducts minimizing the effects of toxicity over a long period. The fate and biochemical implication of these nanoparticles within the cell, however, is complicated and requires additional studies to be used in the best therapeutics. Purpose: The purpose of the study is to examine the intracellular transport, degradation, influence of oxidative stress, and cell viability caused by biodegradable nanoparticles to achieve maximum therapeutic benefit with minimum side effects. Procedure: The biodegradable nanoparticles (PLGA and lipid-based) were produced and analyzed in terms of size, charge, and morphology. The cellular uptake was determined in HeLa and RAW 264.7 macrophage using fluorescence scores and flow cytometry. The measurement of degradation rates was done by use of HPLC and the measurement of the level of oxidative stress by DCFH-DA staining. The MTT assay was used to determine the viability of cells. Results: It was found that the uptake of nanoparticles was time-dependent, with lipid-based nanoparticles exhibiting the greatest internalization. The degradation rate of NP3 (lipid based) was the highest and caused more oxidative stress than NP1 (PLGA). The viability of cells declined substantially with time with NP3 leading to the greatest decline. Conclusion: Biodegradable nanoparticles have time-dependent cellular uptake, degradation and oxidative stress generation, lipid-based nanoparticles have better degradation and greater toxicity. Such results highlight the importance of designing nanoparticles as carefully as possible to maximize therapeutic utility and reduce the negative effects of nanoparticles on cells.