Аңдатпа
Proviral load is a crucial diagnostic indicator that helps determine the level of animal infection and assess the risk of disease progression. A high proviralload is associated with a greater likelihood of clinical manifestations and virus transmission within the herd, making this parameter critically important for veterinary epizootiology. The article presents materials and methods for studying the proviral load of bovine leukemia virus (BLV). The study involved 54 head of cattle. Blood samples were collected using vacuum systems into EDTA tubes. DNA extraction was performed using the commercial kit "Diatom DNA Prep 200," and its concentration was measured with a Qubit 4 fluorometer. Quantitative determination of proviral load was carried out using real-time PCR with the reagent kit "RealBest-Vet DNA of bovine leukemia virus." Primers and probes were used in accordance with the protocol by Marzena Rola-Łuszczaket al. (2004). A standard curve was constructed based on serial dilutions of PCR product (10³–10⁷ copies/reaction). Data analysis was performed using Bio-Rad CFX Maestro Software and Microsoft Excel. Statistical processing included calculations of mean values, standard deviations, and coefficients of variation. The study revealed discrepancies between the results of the commercial bovine leukemia virus detection kit and quantitative PCR, underscoring the need for method standardization to improve diagnostic accuracy. An analysis of correlations between proviral load levels and clinical disease progression was conducted, enabling a differentiated approach to BLV infection diagnosis and control. Proviral load values ranged from 0 copies/100 ng DNA to 192,257.23 copies/100 ng DNA, with an average level of 532,691 copies/100 ng DNA. These findings are significant for developing effective programs to combat bovine leukemia virus