Аңдатпа
Based on the selection of nucleotide sequences of the genome or individual RNA fragments of the virus from the international database at the NCBI website, a large number of sequences for the bovine leukemia virus were identified, which are stored in gene banks and are updated daily with new data. The design of primers in compliance with the required parameters is carried out using various computer programs, the main of which are MUSCLE, UGENE V.36.0, Primer-BLAST, Oligo Analyzer and others. The designed primers were then synthesized on an Expedite 8909 oligonucleotide synthesizer according to the instructions supplied with the device. As a result of the experiments, we have selected and synthesized specific synthetic oligonucleotides PCR_POL_1F and PCR_POL_1R, PCR_POL_2F and PCR_POL_2R, for setting PCR in bovine leukemia.