Аңдатпа
The wide distribution of drug-resistant tuberculosis (TB) remains one of the main healthcare issues in Kazakhstan. The study aims to evaluate the association of drug-resistant TB with the Beijing genotype and different clinical forms among new TB cases in Kazakhstan. 391 clinical isolates of M.tuberculosis were obtained from patients with different clinical TB forms. Drug susceptibility testing (DST) was done for first-line anti-TB drugs using the BACTEC-MGIT 960 system. M.tuberculosis isolates were genotyped using the 24 MIRU-VNTR approach. Statistical analysis was performed by SPSS and MedCalc online. DST results showed that 55,8% (218/391) of isolates were drug-resistant. Among resistant TB cases, 56,4% (123/218) of samples were MDR (resistant to rifampicin and isoniazid). MIRU-VNTR genotyping identified the Beijing genotype in 62,7% (245/391) of cases. In our study, the Beijing genotype was strongly associated with drug-resistant TB (p<0,0001), including MDR-TB (p=0,0002), but did not correlate with any clinical TB forms (p>0,05). Statistical analysis of various clinical TB forms with different forms of drug resistance only indicated an association of focal TB with drug-susceptibility (p=0,0272; OR=0,0950; 95% CI: 0,0118-0,7675). Beijing genotype plays an important role in the distribution of drug-resistant, especially MDR-TB in Kazakhstan. Focal TB is caused by drug-susceptible isolates, mostly by non-Beijing genotypes, which is promising for treatment success. Funding: This research was funded by a grant from Nazarbayev University under Collaborative Research Program №11022021CRP1511, U.K.